ampk cell signaling technology Search Results


93
Cell Signaling Technology Inc ampk antibody sampler kit
AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and <t>AMPK</t> (AMP-activated protein kinase) pathways are involved in DRD3-regulated autophagy. ( A ) DRD3 or DRD5 RNAi in HeLa wild type or HeLa cells stably expressing DRD5-GFP-3FLAG. ( B ) HeLa cells stably expressing GFP-DRD3-3FLAG were treated with increasing concentrations of dopamine for 1 h or 8 h. ( C ) Co-Immunoprecipitation using anti-FLAG in HeLa cells stably expressing GFP-DRD3-3FLAG or GIPC1-GFP-3FLAG treated with Baf A1 and/or NH 4 Cl, in the presence of additional 0.1% Triton X-100 in IP and washing buffers. ( D ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG decreases AMPK activity shown by AMPKα-T172 and β-S108. ( E ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG partially antagonizes the effect of ammonia-induced AMPKα-T172 and β-S108 inhibition. The blue dashed lines are used to distinguish different parts of the results for better visualization. Experiments were repeated at least three times and representative Western blots are shown. Densitometric analysis was performed and quantification results were labeled below the corresponding blots.
Ampk Antibody Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
Cell Signaling Technology Inc phospho
AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and <t>AMPK</t> (AMP-activated protein kinase) pathways are involved in DRD3-regulated autophagy. ( A ) DRD3 or DRD5 RNAi in HeLa wild type or HeLa cells stably expressing DRD5-GFP-3FLAG. ( B ) HeLa cells stably expressing GFP-DRD3-3FLAG were treated with increasing concentrations of dopamine for 1 h or 8 h. ( C ) Co-Immunoprecipitation using anti-FLAG in HeLa cells stably expressing GFP-DRD3-3FLAG or GIPC1-GFP-3FLAG treated with Baf A1 and/or NH 4 Cl, in the presence of additional 0.1% Triton X-100 in IP and washing buffers. ( D ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG decreases AMPK activity shown by AMPKα-T172 and β-S108. ( E ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG partially antagonizes the effect of ammonia-induced AMPKα-T172 and β-S108 inhibition. The blue dashed lines are used to distinguish different parts of the results for better visualization. Experiments were repeated at least three times and representative Western blots are shown. Densitometric analysis was performed and quantification results were labeled below the corresponding blots.
Phospho, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
Cell Signaling Technology Inc anti ampk
AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and <t>AMPK</t> (AMP-activated protein kinase) pathways are involved in DRD3-regulated autophagy. ( A ) DRD3 or DRD5 RNAi in HeLa wild type or HeLa cells stably expressing DRD5-GFP-3FLAG. ( B ) HeLa cells stably expressing GFP-DRD3-3FLAG were treated with increasing concentrations of dopamine for 1 h or 8 h. ( C ) Co-Immunoprecipitation using anti-FLAG in HeLa cells stably expressing GFP-DRD3-3FLAG or GIPC1-GFP-3FLAG treated with Baf A1 and/or NH 4 Cl, in the presence of additional 0.1% Triton X-100 in IP and washing buffers. ( D ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG decreases AMPK activity shown by AMPKα-T172 and β-S108. ( E ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG partially antagonizes the effect of ammonia-induced AMPKα-T172 and β-S108 inhibition. The blue dashed lines are used to distinguish different parts of the results for better visualization. Experiments were repeated at least three times and representative Western blots are shown. Densitometric analysis was performed and quantification results were labeled below the corresponding blots.
Anti Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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94
Cell Signaling Technology Inc mouse anti acc
AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and <t>AMPK</t> (AMP-activated protein kinase) pathways are involved in DRD3-regulated autophagy. ( A ) DRD3 or DRD5 RNAi in HeLa wild type or HeLa cells stably expressing DRD5-GFP-3FLAG. ( B ) HeLa cells stably expressing GFP-DRD3-3FLAG were treated with increasing concentrations of dopamine for 1 h or 8 h. ( C ) Co-Immunoprecipitation using anti-FLAG in HeLa cells stably expressing GFP-DRD3-3FLAG or GIPC1-GFP-3FLAG treated with Baf A1 and/or NH 4 Cl, in the presence of additional 0.1% Triton X-100 in IP and washing buffers. ( D ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG decreases AMPK activity shown by AMPKα-T172 and β-S108. ( E ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG partially antagonizes the effect of ammonia-induced AMPKα-T172 and β-S108 inhibition. The blue dashed lines are used to distinguish different parts of the results for better visualization. Experiments were repeated at least three times and representative Western blots are shown. Densitometric analysis was performed and quantification results were labeled below the corresponding blots.
Mouse Anti Acc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Cell Signaling Technology Inc ampk substrate antibody sampler kit
AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and <t>AMPK</t> (AMP-activated protein kinase) pathways are involved in DRD3-regulated autophagy. ( A ) DRD3 or DRD5 RNAi in HeLa wild type or HeLa cells stably expressing DRD5-GFP-3FLAG. ( B ) HeLa cells stably expressing GFP-DRD3-3FLAG were treated with increasing concentrations of dopamine for 1 h or 8 h. ( C ) Co-Immunoprecipitation using anti-FLAG in HeLa cells stably expressing GFP-DRD3-3FLAG or GIPC1-GFP-3FLAG treated with Baf A1 and/or NH 4 Cl, in the presence of additional 0.1% Triton X-100 in IP and washing buffers. ( D ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG decreases AMPK activity shown by AMPKα-T172 and β-S108. ( E ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG partially antagonizes the effect of ammonia-induced AMPKα-T172 and β-S108 inhibition. The blue dashed lines are used to distinguish different parts of the results for better visualization. Experiments were repeated at least three times and representative Western blots are shown. Densitometric analysis was performed and quantification results were labeled below the corresponding blots.
Ampk Substrate Antibody Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ampk+cell+signaling+technology/AMPK+Substrate+Antibody+Sampler+Kit/pm40663935-107-60-65
Average 93 stars, based on 1 article reviews
ampk substrate antibody sampler kit - by Bioz Stars, 2026-09
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Cell Signaling Technology Inc anti cnpase
AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and <t>AMPK</t> (AMP-activated protein kinase) pathways are involved in DRD3-regulated autophagy. ( A ) DRD3 or DRD5 RNAi in HeLa wild type or HeLa cells stably expressing DRD5-GFP-3FLAG. ( B ) HeLa cells stably expressing GFP-DRD3-3FLAG were treated with increasing concentrations of dopamine for 1 h or 8 h. ( C ) Co-Immunoprecipitation using anti-FLAG in HeLa cells stably expressing GFP-DRD3-3FLAG or GIPC1-GFP-3FLAG treated with Baf A1 and/or NH 4 Cl, in the presence of additional 0.1% Triton X-100 in IP and washing buffers. ( D ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG decreases AMPK activity shown by AMPKα-T172 and β-S108. ( E ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG partially antagonizes the effect of ammonia-induced AMPKα-T172 and β-S108 inhibition. The blue dashed lines are used to distinguish different parts of the results for better visualization. Experiments were repeated at least three times and representative Western blots are shown. Densitometric analysis was performed and quantification results were labeled below the corresponding blots.
Anti Cnpase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ampk+cell+signaling+technology/PTMScan+Phospho-AMPK+Substrate+Motif+(LXRXXS*%2FT*)+Kit/pm36464774-106-32-37
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Image Search Results


AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and AMPK (AMP-activated protein kinase) pathways are involved in DRD3-regulated autophagy. ( A ) DRD3 or DRD5 RNAi in HeLa wild type or HeLa cells stably expressing DRD5-GFP-3FLAG. ( B ) HeLa cells stably expressing GFP-DRD3-3FLAG were treated with increasing concentrations of dopamine for 1 h or 8 h. ( C ) Co-Immunoprecipitation using anti-FLAG in HeLa cells stably expressing GFP-DRD3-3FLAG or GIPC1-GFP-3FLAG treated with Baf A1 and/or NH 4 Cl, in the presence of additional 0.1% Triton X-100 in IP and washing buffers. ( D ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG decreases AMPK activity shown by AMPKα-T172 and β-S108. ( E ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG partially antagonizes the effect of ammonia-induced AMPKα-T172 and β-S108 inhibition. The blue dashed lines are used to distinguish different parts of the results for better visualization. Experiments were repeated at least three times and representative Western blots are shown. Densitometric analysis was performed and quantification results were labeled below the corresponding blots.

Journal: International Journal of Molecular Sciences

Article Title: Dopamine Receptor Subtypes Differentially Regulate Autophagy

doi: 10.3390/ijms19051540

Figure Lengend Snippet: AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and AMPK (AMP-activated protein kinase) pathways are involved in DRD3-regulated autophagy. ( A ) DRD3 or DRD5 RNAi in HeLa wild type or HeLa cells stably expressing DRD5-GFP-3FLAG. ( B ) HeLa cells stably expressing GFP-DRD3-3FLAG were treated with increasing concentrations of dopamine for 1 h or 8 h. ( C ) Co-Immunoprecipitation using anti-FLAG in HeLa cells stably expressing GFP-DRD3-3FLAG or GIPC1-GFP-3FLAG treated with Baf A1 and/or NH 4 Cl, in the presence of additional 0.1% Triton X-100 in IP and washing buffers. ( D ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG decreases AMPK activity shown by AMPKα-T172 and β-S108. ( E ) DRD3 RNAi in HeLa cells stably expressing GFP-DRD3-3FLAG partially antagonizes the effect of ammonia-induced AMPKα-T172 and β-S108 inhibition. The blue dashed lines are used to distinguish different parts of the results for better visualization. Experiments were repeated at least three times and representative Western blots are shown. Densitometric analysis was performed and quantification results were labeled below the corresponding blots.

Article Snippet: The autophagy antibody sampler kit, the antibodies for phospho-S6K (T389/412), S6K, AKT pan, phospho-AKT (Thr-308), phospho-AKT (Ser-473), mTOR, AMPK Antibody sampler kit, the HRP-linked anti-rabbit and anti-mouse IgG antibody were all from Cell signaling technology.

Techniques: Stable Transfection, Expressing, Immunoprecipitation, Activity Assay, Inhibition, Western Blot, Labeling